Ready-To-Use Cell-based Permeability System

We deliver a number of different AssayReady™ Cell-based Permeability Systems which offer a differentiated epithelial cell barriers that can be easily and directly used in permeability measurement and transport of drugs into tissues as well as across cell membranes, including intestinal, airway, ocular, oral absorption and BBB permeation.

Our AssayReady™ Cell-based Permeability System consists of T84 (intestinal epithelial), MDR-1/MDCK (kidney epithelial transfected with MDR1 gene), and Calu3 (airway epithelial) permeability systems in multi-well plate formats (6-, 12-, 24-, 96 well plate). These time saving and cost-effective permeability systems are ready to use and adaptable to high throughput screening. Our unique technology ensures high stability of differentiated cell barrier and maintains full cell functionality after transportation.

Benefits include:
- No more need for continuous cell culture
- Ready to Use
- Easy handling
- High stability of cell barriers which maintain full cell functionality for your studies
 

AssayReady™ T84 Intestinal Epithelial Barrier Permeability System

T84 cells can differentiate into a highly functionalized epithelial barrier with remarkable morphological and biochemical similarity to the small intestinal columnar epithelium. The utilization of T84 cells has become an industry standard for the investigation of intestinal absorption, permeability and barrier function study. MB Biosciences supplies AssayReady™ T84 Intestinal Epithelial Barrier for the understanding of molecular composition and regulation of intestinal barrier function.

MB Biosciences offers AssayReady™ T84 Intestinal Epithelial Barrier in the following plated formats: 
    6-well, 12-well, 24-well, 96-well

Upon receipt, AssayReady™ T84 Epithelial Barrier requires minimal handling before beginning the assay.

AssayReady™ Caco-2 Intestinal Epithelial Barrier Permeability System

MB Biosciences supplies AssayReady™ Caco-2 [Caco2] Intestinal Epithelial Barrier for drug discovery applications as part of our AssayReady™ Products. Ready to use 21-day differentiated Caco-2 cell monolayer allows customers to decouple cell culture preparation from the evaluation of in vitro intestinal absorption of drug targets.

MB Biosciences offers AssayReady™ Caco-2 Epithelial Barrier in the following plated formats:
    6-well, 12-well, 24-well, 96-well

Upon receipt, AssayReady™ Caco-2 Epithelial Barrier requires minimal handling before beginning the assay.

AssayReady™ P-gp-induced Caco-2 Epithelial Barrier Permeability System

P-gp protein expression can be induced in Caco2 cell monolayer by several drugs such as vinblastine (VinB). MB Biosciences supplies AssayReady™ P-gp-induced Caco-2 epithelial barrier for drug discovery applications as part of our AssayReady™ Products. Ready to use 21-day differentiated P-gp-induced Caco-2 cell monolayer allows customers to decouple cell culture preparation from the evaluation of P-gp-mediated transport.

MB Biosciences offers AssayReady™ P-gp-induced Caco-2 Epithelial Barrier in the following plated formats:
    6-well, 12-well,  24-well,  96-well

Upon receipt, AssayReady™  P-gp-induced Caco-2 Epithelial Barrier requires minimal handling before beginning the assay.

AssayReady™ MDR1-MDCK Epithelial Barrier Permeability System

Madin-Darby canine kidney (MDCK) cells transfected with human MDR1 gene (MDCK-MDR1) encoding for P-glycoprotein (hP-gp, ABCB1) are widely used for transport studies to identify drug candidates as substrates of this efflux protein. Using AssayReady™ MDR1-MDCK Permeability System avoids the complexities of multiple transporters by focusing specifically on P-gp, one of the most well-recognized efflux transporters in many tissues including the brain, kidney, and intestine. This ready to use permeability system helps to gain a greater understanding of the mechanism of drug efflux and highlights early potential issues with drug permeability. 

MB Biosciences offers AssayReady™ MDR1-MDCK Epithelial Barrier in the following plated formats:
    6-well, 12-well, 24-well, 96-well

MB Biosciences also offers AssayReady™ MDCK Epithelial Barrier in the in the following plated formats:
    6-well, 12-well, 24-well, 96-well

Upon receipt, AssayReady™ MDR1-MDCK or MDCK Permeability Barriers require minimal handling before beginning the assay.

AssayReady™ Calu3 Airway Epithelial Barrier Permeability System

MB Biosciences supplies AssayReady™ Calu3 Airway Epithelial Barrier for studying the effects of gas concentrations, exposure time, biophysical stress, and biological agents on human airway barrier function as part of our AssayReady™ Products. Ready to use differentiated human Calu3 Airway epithelial cell barrier is a good model in respiratory study.

MB Biosciences offers AssayReady™ Calu3 Airway Epithelial Barrier in the following plated formats:
    6-well, 12-well,  24-well, 96-well

Upon receipt, AssayReady™ Calu3 Airway Epithelial Barrier requires minimal handling before beginning the assay.

AssayReady™ Permeability Kit  

A convenient set of buffers, and reagents are at a value price. AssayReady™ Permeability Kit was specifically developed for cell-based permeability assay which includes assay buffers (transport buffer) and permeability markers (Lucifer yellow).