HEPSPAN™ LONG-TERM CULTURE PRIMARY HEPATOCYTE MEDIUM AND HEPSPAN™ LONG-TERM CULTURE PRIMARY HEPATOCYTE

HEPSPAN™ LONG-TERM CULTURE PRIMARY HEPATOCYTE MEDIUM

Current standard monolayer hepatocyte cultures are typically functional in culture for 5 to 7 days. There is an increasing need for longer term cultures of hepatocytes to study liver toxicity, drug metabolism, drug interactions, viral infection, inflammation, liver development and regeneration. In order to extend the functional life of hepatocytes in culture, we have developed HepSpan™ medium, a long-term culture of primary hepatocyte medium which maintains the morphology and function of primary hepatocyte in standard 2D cultures for 4 weeks (human hepatocytes), 3 weeks (rat hepatocytes) and 2 weeks (mouse hepatocytes).

HepSpan™ medium is optimized for primary hepatocyte to enhance cell viability, function and long-term maintenance. HepSpan™ is a better representation of the liver’s extracellular environment for the initiation and maintenance of primary hepatocytes long-term cultures.

HepSpan™ Medium Advantages

• Better representation of the liver’s extracellular environment

• Improved hepatocytes function and high levels of liver-specific gene expression

• Stable albumin and urea secretion for four weeks in culture

• Maintain Long-term CYP activities and CYP inducibility of primary hepatocytes

• Support long-term culture of cryopreserved and fresh plateable primary hepatocytes 

HepSpan™ Medium Features

• Defined medium tailored to support hepatocyte long term in vitro culture 

• Adjusted to standard 2D monolayer culture and high throughput

• Raw material screening and quality control ensure minimal lot-to-lot variability

• Multi-species culture medium available (Human, Rat, Mouse, Dog, Monkey)

HepSpan™ Medium Application

• Hepatic Disease Model

• Drug Metabolism

• Metabolic Disorders Study

• Drug induced liver injury (DILI) Study

• Drug Transporters and Drug-Drug Interaction

Assessment of 4 weeks cultured Hepatic Functions of Human Hepatocytes utilizing HepSpan™ Media

HepSpan™ Maintenance Medium

HepSpan™ Ready-to-Use Long-Term Cultured Primary Hepatocyte

Primary hepatocytes and their adherent cultures are the gold-standard in vitro tool to evaluate liver toxicity, drug metabolism, cytochrome P450 induction, drug-drug interactions, viral infection, inflammation, liver development and regeneration.  However, primary hepatocytes rapidly lose their morphology and liver-specific functions, such as detoxification, activity of phase I and phase II enzymes, and the production of plasma proteins like albumin over a few days in standard culture. 

MB Biosciences also provide HepSpan™ Ready-to-use long term cultured primary hepatocytes which include a pre-seeded hepatocytes in multi-well formats plates with long-term culture medium to overcome the short stability culture problem as well as standard issues of working with primary hepatocyte such as hepatocyte plating failure, poor viability, and alleviates any unnecessary time and cost spent on finding exceptional lots.

HepSpan™ ready-to-use cultured primary hepatocytes with a prolonged culture up to 4 weeks for human hepatocytes, 3 weeks for rat hepatocytes and 2 weeks for mouse hepatocytes in standard 2D-culture formats (24 wells, 48 wells, 96 wells and 384 wells).

HepSpan™ ready-to-Use Hepatocytes is an innovative platform for ADME/TOX, viral infection, inflammation and liver biology study. They are stored and transported at room temperature, and enables the ability to perform the experiments that require a longer time culture of primary hepatocytes.

HepSpan™ Ready-to-Use Long-Term Cultured Primary Hepatocyte Features

• Ready-To-Use for your assay right after delivery

• High quality pre-screened human primary hepatocytes and fresh animal hepatocytes

• Full Hepatocytes functionality after transportation

• Maintain Long-term CYP activities and CYP inducibility of primary hepatocytes

• Stable albumin and urea secretion for four weeks in culture

• Don't need Matrigel overlays, complicated 3D culture systems or bioreactors

HepSpan™ Ready-to-Use Long-Term Cultured Primary Hepatocyte Benefits

• Time saving and cost-effective

• Not employ genetically modified human hepatocyte cells or cell lines.

• High levels of liver-specific gene expression, metabolic and synthetic function 

• Easily adaptable to automated procedures and HTS 

• Multi-species available (Human, Rat, Mouse, Dog, Monkey)