In Vitro Services
MB Biosciences provides a wide range of standardized and customized contract assay services for clients in pharma, biotech and other life sciences organizations. Our expertise covers many aspects of primary cell biology with a special emphasis on Hepatic (Hepatocytes and Non-parenchymal liver cell) primary cell systems. We assist clients with early stage research projects through multiple areas of pre-clinical and clinical drug development.
Custom Hepatocyte And Non-parenchymal Liver Cell Isolation
MB Biosciences can be your partner for custom isolation of hepatocytes or primary cells from your animals or from selected rodent strains and transgenic animals. We perfusion, isolate, preserve and cryopreserve primary cells for shipment to the customer. You simply plate and use. You receive primary cells in suspension format or in the following plated formats that you desire, together with documentation of characterization. Highly trained staff works closely with you at every step to ensure your satisfaction.
Hepatocyte Isolation
Hepatocytes are isolated from mouse and rat liver by two step of collagenase perfusion. Cells can be purified by density gradient centrifugation or centrifugal elutriation. Cells are suitable for use in suspension or can be plated in primary culture.
Non-parenchymal liver cell isolation
The MB Biosciences provide preparation of hepatic stellate cells, sinusoidal endothelial cells and Kupffer cells from rats and mice. The procedure involves collagenase perfusion of the liver followed by a cell- and species-specific combination of purification steps. Cells are suitable for use in suspension or can be plated in primary culture.
3D Hepatocytes Culture and Hepatic Cell Co-Cultures
Hepatocyte cultured together with liver non-parenchymal cells such as sinusoidal hepatic endothelial cells, Kupffer cells and hepatic stellate cells are powerful in vitro tools to model normal and inflamed liver states.
MB Biosciences’ scientists have extensive experience in using a Hepatocyte and liver non-parenchymal cells 3D co-culture systems to test the effects of test compounds on certain cell types. Ability to model normal and inflamed liver states - make your own custom ratios.
Caco-2 Cell Monolayer Model Cell Culture
MB Biosciences works as an extension of a customer’s team to provide Caco-2 cell monolayer culture services that can be time saving and cost-effective for your oral absorption, bioavailability evaluation, drug transporter and Drug-Drug interaction studies.
MB Biosciences scientists are experts in the Caco-2 cell monolayer model, a combination of experience and rigorous cell biology QC processes assures high stability of Caco-2 barrier and maintains full cell functionality for your studies.
MB Bio’s Caco-2 cell services include expert scientific consultation to plan your Caco-2 monolayer culture, fast turn-around with scheduled status updates of your cells. The Caco-2 cells are seeded in 24 and 96-well Caco-2 insert plates. Caco-2 cells are cultured 21 days after cell seeding to allow for complete monolayer formation and cell differentiation. Prior to delivery to your laboratory, the integrity of Caco-2 monolayers are verified by transepithelial electrical resistance (TEER) measurements.
Liver-specific Assays Services
Hepatocyte cultures are regarded as the gold standard in cell culture techniques for the evaluation of hepatic drug metabolism pharmacokinetics, toxicity and other xenobiotics as well as biological, pharmacological and toxicological studies.
MB Biosciences offers a range of in vitro Liver-specific assays services using our freshly isolated hepatocytes, sandwich cultured hepatocytes and 3D liver microtissues. Our goal is providing our services at fast turn-around-times by optimizing the methods and sample extraction procedures, for quantitative as well as qualitative analysis. MB Biosciences Team can meet your needs in all phases of the drug discovery and development process.
We offer customers fee-for-services for Liver-specific assays at our Laboratories for the following:
• Hepatocyte Uptake Assay
• Hepatocyte clearance assay
• Hepatocyte Biliary Excretion Assay
• Hepatocyte Functional polarity assay
• Hepatic phospholipidosis assay
• Hepatic cholestasis assay
• 3D Hepatocyte Clearance – with specific benefits for study of low clearance compounds
• Met ID
• CYP induction/inhibition
• Toxicology and Mechanisms of Action
• Inflammation-mediated toxicity
Bioassay Services
MB Biosciences offers a comprehensive functional in-vitro cell-based assay platform for your drug development needs. Our Bioassay Services include:
Cell proliferation and survival assay
Apoptosis assay
Cell senescence assay
Angiogenesis assay
Cell migration and invasion assays
Primary cell immobilization service
Stable and transient gene expression and knockdown service
We are continually developing new assays and would be pleased to add others upon your request.