HepSpan™ Ready-to-Use Long-Term Cultured Primary Hepatocyte System

Primary hepatocytes and their adherent cultures are the gold-standard in vitro tool to evaluate liver toxicity, drug metabolism, cytochrome P450 induction, drug-drug interactions, viral infection, inflammation, liver development and regeneration.  However, primary hepatocytes rapidly lose their morphology and liver-specific functions, such as detoxification, activity of phase I and phase II enzymes, and the production of plasma proteins like albumin over a few days in standard culture. 

MB Biosciences provide HepSpan™ primary hepatocytes to overcome the short-term culture problem, as well as problems of working with primary hepatocyte such as hepatocyte plating failed, poor viability, time consuming and costly for better lot selecting. 

HepSpan™ Hepatocytes is ready-to-use cultured primary hepatocytes with a prolonged culture up to 4 weeks for human hepatocytes, 3 weeks for rat hepatocytes and 2 weeks for mouse hepatocytes.

HepSpan™ Primary hepatocyte maintains primary hepatocyte viability, liver specific functions and typical morphology for several weeks in standard 2D-culture formats (24 wells, 48 wells, 96 wells and 384 wells).

HepSpan™ ready-to-Use Hepatocytes is an innovative platform for ADME/TOX, viral infection, inflammation and liver biology study. They are stored and transported at room temperature, and allow to perform the experiments that need longer time culture of primary hepatocytes.

HepSpan™ Application

• Drug Metabolism

• Hepatic Disease Model

• Metabolic Disorders Study

• Drug induced liver injury (DILI) Study

• Drug Transporters and Drug-Drug Interaction

HepSpan™ Features

• Ready-To-Use for your assay

• High quality pre-screened human primary hepatocytes and fresh animal hepatocytes

• Full Hepatocytes functionality after transportation

• Maintain Long-term CYP activities and CYP inducibility of primary hepatocytes

• Stable albumin and urea secretion for four weeks in culture

• Don't need Matrigel overlays, complicated 3D culture systems or bioreactors

HepSpan™ Benefits

• Time saving and cost-effective

• Not employ genetically modified human hepatocyte cells or cell lines.

• High levels of liver-specific gene expression, metabolic and synthetic function 

• Easily adaptable to automated procedures and HTS 

• Multi-species available (Human, Rat, Mouse, Dog, Monkey)

Assessment of 4 weeks cultured Hepatic Functions of HepSpan™ primary human hepatocytes

HepSpan™ Ready-To-Use Primary Hepatocytes

HepSpan™ Maintenance Medium