HepSpan™ Long-Term Culture of Primary Hepatocyte Medium

Current standard monolayer human hepatocyte cultures are typically functional in culture for 5 to 7 days. There is an increasing need for longer term cultures of hepatocytes to study liver toxicity, drug metabolism, drug interactions, viral infection, inflammation, liver development and regeneration. To extend the functional life of hepatocytes in culture, we develop HepSpan™ medium, a long-term culture of primary hepatocyte medium kit which maintains the morphology and function of primary hepatocyte in standard 2D cultures for 4 weeks (human hepatocytes), 3 weeks (rat hepatocytes) and 2 weeks (mouse hepatocytes).

HepSpan™ medium kit is optimized for primary hepatocyte to enhance cell viability, function and long-term maintenance. HepSpan™ is more representative of the liver’s extracellular environment for the initiation and maintenance of primary hepatocytes long-term cultures.

HepSpan™ Medium Advantages

• More representative of the liver’s extracellular environment

• Improved hepatocytes function and high levels of liver-specific gene expression

• Stable albumin and urea secretion for four weeks in culture

• Maintain Long-term CYP activities and CYP inducibility of primary hepatocytes

• Support long-term culture of cryopreserved and fresh plateable primary hepatocytes 

• Don’t need co-culturing of human hepatocytes with other species of cells

• Don't need Matrigel overlays, complicated 3D culture systems or bioreactors

HepSpan™ Medium Benefits

• Defined medium tailored to support hepatocyte long term in vitro culture 

• Adjusted to standard 2D monolayer culture and high throughput

• Raw material screening and quality control ensure minimal lot-to-lot variability

• Multi-species culture medium available (Human, Rat, Mouse, Dog, Monkey)

HepSpan™ Medium Application

• Hepatic Disease Model

• Drug Metabolism

• Metabolic Disorders Study

• Drug induced liver injury (DILI) Study

• Drug Transporters and Drug-Drug Interaction

HepSpan™ Maintenance Medium